H3F3A(组蛋白3.3)G34W免疫组化-一个用于确定骨巨细胞瘤良恶性的可靠标志物
  ——本文经《美国外科病理学杂志》授权发布,其他媒体转载或引用须经《美国外科病理学杂志》同意,否则追究法律责任。

  摘要
  骨巨细胞瘤(giant cell tumor of bone,GCTB)是一种发生于关节下、具有局部侵袭性的肿瘤。最近报道称,H3.3 G34W突变是这类肿瘤的特征性改变,我们研究了抗组蛋白H3.3 G34W兔单克隆抗体在多种肿瘤中的敏感性和特异性,其中包括形态学上易与GCTB混淆的肿瘤,以评估其作为诊断性标志物的价值。我们另外通过评价基因表型和H3.3 G34W免疫染色结果来判定H3.3 G34W突变在原发恶性骨肿瘤中的发生率。我们共测试了3163例肿瘤。最终,H3.3 p.G 34W抗体在235例GCTB中有213例(90.6%)细胞核阳性表达。但是,H3.3 p.G34L、M、V等罕见亚型并非如此,它们往往发生于手骨、髌骨、中轴骨。如果剔除这些部位,H3.3 p.G34W抗体在GCTB中的阳性表达率高达97.8%。恶性骨肿瘤中最初划分为骨肉瘤的部分(n=11)是唯一G34W抗体阳性表达的其它病变。值得注意的是,先前有两篇报道称,伴有p.G34R突变的骨肉瘤对于该抗体并不具有免疫反应性。另外,13例伴有H3.3突变的恶性骨肿瘤中有11例形态学上具有富于破骨细胞的区域,并且影像学证实除1例以外,其余均发生于关节下。因此,我们认为,发生于关节下并伴有H3.3突变的原发恶性骨肉瘤,即便缺乏良性GCTB成分,也可以诊断为真正的恶性GCTB。
 
  来源:Am J Surg Pathol 2017;41:1059–1068
  美国外科病理学杂志中文版2017年第一期摘要No.4
  (倪浩 翻译;郭凌川 审校)
  The American Journal of Surgical Pathology中文版声明:
  2017 American Journal of Surgical Pathology and Wolters Kluwer Health
  The material is published by Wolters Kluwer Health with the permission of American Journal of Surgical Pathology.No
  part of this publication may be reproduced in any form,stored in a retrieval system or transmitted in any form,by any means,without prior written permission from Wolters Kluwer Health.Opinions expressed by the authors and advertisers are not necessarily those of the American Journal of Surgical Pathology, its affiliates,or of the Publisher.The American Journal of Surgical Pathology,its affiliates,and the Publisher disclaim any liability to any party for the accuracy,completeness,efficacy,or availability of the material contained in this publication (including drug dosages) or for any damages arising out of the use or non-use of any of the material contained in this publication.
  Although advertising material is expected to conform to ethical (medical) standards,inclusion in this publication does not constitute a guarantee or endorsement of the quality or value of such product or of the claims made of it by its manufacturer.
 
  【本文经《美国外科病理学杂志》授权发布,其他媒体转载或引用须经《美国外科病理学杂志》同意,否则追究法律责任;所有文章仅供公益交流,不代表本站立场。欢迎提供素材、资料等,投稿邮箱:tougao@91360.com,一经采纳将给予稿费】